Research

Developing Tools for the Engineering of Fungi

Fungi represent an incredibly rich reservoir of natural products, which often show potent bioactivity and find applications in different fields. Research in the Alberti Lab aims to develop new synthetic biology tools that can be employed to engineer higher fungi and explore the biosynthetic diversity that they have to offer. 

Selected reference:​

• Alberti F et al. (2020) Biol Open 9, bio056010.

Current group members working on this theme:

• Dr Panward Prasongpholchai (PDRA)

• Dr Tiantian Fu (Senior Research Technician)

In our group, we aim to discover natural products from fungi, including ascomycete and basidiomycete strains. For instance, we have recently characterised, in collaboration with the Bunawan lab (National University of Malaysia), Trichoderma strains that inhibit the growth of the papaya dieback pathogen Erwinia mallotivora. We are currently working on the isolation of natural products involved in the antimicrobial activity of the fungi.

We also work on actinomycetes bacteria. In collaboration with the Corre Lab (Warwick), we optimised a strategy to discover natural products from actinomycetes and characterise their biosynthesis. In this approach, gene clusters are captured through yeast-based homologous recombination, and biosynthetic genes are derepressed with CRISPR/Cas9-mediated genome editing.

Selected references:

• Tamizi A-A et al. (2022) J Fungi 8 (3): 246. 

• Alberti F et al. (2019) Chem Sci 10(2): 453-463.

• Alberti F and Corre C (2019) Nat Prod Rep, 36: 1237-248

Current group members working on this theme:

• Mr Richard Olumakaiye (PhD student)

• Ms Sophie Jin (PhD student)

• Ms Jingfan Zhang (PhD student)